RHIZO-BIODEGRADATION OF METHYLENE BLUE DYE USING DEVELOPED MYCORRHIZAL SOILPoonam Pal and Hardik PatelAbstract The dyestuff manufacturing industry has been categorized as one of the hazardous industry by EPA under Hazardous Waste Management and Handling Rules (1989). Methylene blue is one of the commonly found compounds in hazardous waste. The present research emphasizes development of Rhizosphere bioremediation of dye compound â Methylene blue in pot culture using developed mycorrhizal soil which was grown for 3 months. Then bioremediation was carried out in mycorrhizal amended soil with dye compound of varying concentration (0, 10, 25, 50 mg/kg) for another 3 months. Rhizosphere biodegradation of Methylene blue dye was assessed by HPLC and GCMS technique in the lab where degradation in the structure of methylene blue dye was seen by formation of different metabolites in the soil which was confirmed by GCMS and 60% reduction in the quantity of dye was observed in 10 mg pot, 62% dye reduction in 25 mg pot and 64% dye degraded was seen in 50 mg pot by HPLC analysis. Hence the study focuses on formation of mycorrhizal soil which can be used as biofertilizers as well as will help to develop ecofriendly, cost-effective and efficient approaches for decontamination of recalcitrant dye compound from the soil. |