Ecology, Environment and Conservation Paper

Vol 28, Jan Suppl. Issue, 2022; Page No.(68-72)

PREVALENCE AND ANTIBIOTIC RESISTANCE OF STAPHYLOCOCCUS AUREUS ISOLATED FROM ETTAWA CROSSBREED GOAT RAW MILK ON WONOSARI SUB-DISTRIC, MALANG, INDONESIA

Anugrah Aditya Putra, Budiarto, Tita Damayanti Lestari, Mustofa Helmi Effendi, Wiwiek Tyasningsih4 and Sheila Marty Yanestria

Abstract

This research was aimed to determine the prevalence and profile of antibiotic resistant of Staphyloccocus aureus bacteria collected from Ettawa crossbreed goat raw milk at Bagelan farm in Wonosari, Malang, East Java. A total 100 raw milk samples were collected from Bagelan farm. Isolation and identification of Staphyloccocus aureus (S. aureus) were used Mannitol Salt Agar(MSA) media, Gram staining, catalase test, and coagulase test. The antibiotic sensitivity test was done by using diffusion disc Kirby-Bauer method. The results showed that 26 isolates were confirmed as S. aureus. The antibiotic sensitivity test result showed about 4 samples (15%) resistance to cloramfenicol, 5 samples (19%) resistance to tetracyclin, and 14 samples (54%) resistance to erythromycin. S. Aureus were sensitive to Gentamicin and cefoxitin antibiotics (100%). About 1 sample was Multiple Drug Resistance (MDR) on antibiotic tetracyclin, erythromycin, and cloramfenicol. In conclusion, the presence of antibiotic resistant and MDR of S. aureus in Ettawa crossbreed goat raw milk should raise public awareness about the dangers posed by these isolates to spread and threaten human health.