CHARACTERIZATION OF PATHOGENESIS-RELATED TRANSCRIPTIONAL ACTIVATOR GENES (PTI5 AND PTI6) FROM MUSA ACUMINATA (A GENOME) AND MUSA BALBISIANA (B GENOME) USING IN SILICO APPROACHIndah Nurharuni, Fenny M. Dwivany and Karlia Meitha
Bananas plant are perennial monocotyledonous herbs which domestication process started some in Southeast Asia. Most edible bananas belong to the Eumusa (or Musa) section, the majority of these bananas originated from hybridization between M. acuminata and M. balbisiana species. Pathogenesis-Related Genes Transcriptional Activator (PTI5 and PTI6) are proteins that have function in disease resistant in banana plants. With the availability of Musa acuminata, Musa balbisiana genome data, this study aims to identify and compare PTI5 and PTI6 genes in B genome with the annotated model in A genome. The PTI5 and PTI6 genes in A genome were obtained from the National Center of Biotechnology Information (NCBI) and genome B from the analysis of the Basic Local Alignment Search Tool Nuclotide (BLASTN) at Banana Genome Hub. The gene structure, location of exons and introns was predicted using FGENESH+ program. Amino acid sequences were identified for their domains and motifs using the CD-Search Tool and MEMEsuite program. Phylogenetic tree was constructed using protein sequences using MEGAX. The nucleotide sequences and protein sequences Musa acuminata and Musa balbisiana showed similar nucleotide compositions and gene structures with percentage identity 97% for PTI5 and 89% for PTI6. Protein sequences have conserved domain namely APATELA2/Etylene-Responsive Factor (AP2/ERF) with YRG and RAYD motifs which have function as DNA-binding transcription factor fo Pathogenesis-Related genes. The result of this study can be used as a prelimary data for future studies of disease resistant related genes in Musa acuminata and Musa balbisiana.