Ecology, Environment and Conservation Paper

COMPARISON OF ANTIOXIDANT POTENTIAL OF GYNURA PROCUMBENS ADVENTITIOUS ROOT IN VITRO CULTURE AND EX VITRO

Sugiharto, Dannis Yuda Kusuma, Dwi Winarni, Idqa Nurtri Bhakti, Abdul Hakim Muhsyi and Yosephine Sri Wulan Manuhara

Abstract

The aim of this research was to compare antioxidant potential from methanol extract of G. procumbens adventitious root in vitro culture and ex vitro. Adventitious root in vitro biomass was obtained from root harvesting after 28 days of culture in liquid MS medium supplemented with 5 mg/l indole butric acid (IBA) in ballon type bubble bioreactor. Extraction was carried out by the maceration method with methanol as a solvent. The antioxidant potency assay were carried out by determined total phenolic content (TPC), total flavonoid content (TFC), analysis of flavonoids by High Performance Liquid Chromatography (HPLC), and analysis of antioxidant activity by 2,2-Diphenyl-picrylhydrazyl (DPPH) assay. Based on the data analyzed of TPC, TFC, and HPLC assay, they showed that methanol extract of adventitious root in vitro culture and ex vitro of G. procumbens, have potential as an antioxidant activity. Both in vitro and ex vitro the root of G. procumbens contain phenolic and flavonoids compound such as myricetin, kaempherol, quercetin, and catechin. Analysis by DPPH assay, G. procumbens adventitious root in vitro culture indicated moderate antioxidant activity and ex vitro was strong antioxidant activity.