INFLUENCE OF PHYTOHORMONES ON ADVENTITIOUS SHOOT REGENERATION FROM LEAF EXPLANTS OF AN ENDANGERED HIMALAYAN MEDICINAL PLANT SWERTIA CHIRAYITA BUCH.- HAMS. EX WALLGarima Kumari, Ashish Guleria and Kamlesh KanwarAbstract A valuable and regenerative protocol of micropropagation with high efficiency was developed through direct organogenesis from leaf explants under in vitro conditions. Murashige and Skoog (MS) medium supplemented with various growth hormones combinations resulted in maximum 84.26% of shoot bud induction and 88.91% of multiplication. Swertia plants are very difficult to hardened with maximum percent survival upto acclimatization and field transfer. Keeping in view for better root induction microshoots were dipped in 1.0 mg/l IBA for 24 hours and then transferred to half strength MS basal medium supplemented with 0.02% activated charcoal resulted 75.21% rooting with roots having a very good growth. Rooted plantlets were hardened gradually in different potting mixtures and then were successfully acclimatized under ex vitro conditions. |