Pollution Research Paper

BIOLOGICAL CONTROL OF MICROBIAL CONTAMINATION ASSOCIATED WITH PLANT TISSUE CULTURE OF DATE PALM (PHOENIX DACTYLIFERA)

AHMED ABD BURGAL, EMAN ABOOBMUKHAIFI, KHUDHAIR M. ALKANANY AND WIJDAN HUSSEIN AL-TAMIMI

Abstract

Biotechnology in the agriculture field provides tremendous potential for producing crops with lower costs and increased productivity. This is a very essential choice for introducing the best tools of biotechnology to overcome the challenges in this field. In this studyeight bacteria isolate from the contaminated tissue culture of date palm, all isolates were genetically identified based on the 16SrRNA gene. The bacterial strains were Enterococcus faecium strain MK748256 (IW21), Bacillus cereus strain B26 (IW22), Bacillus sp. strain PK-7 (IW24), Bacillus cereus strain JS22 (IW25), Lysinibacillus sp. Strain IADCASC12 (IW28), Lysinibacillus sp. strain IADCASC11 (IW29), Bacillus thuringiensis strain Sol 1 (IW31) and Bacillus cereus strain Q1 (IW32). In the current study, the genus Lysinibacillus was isolated for the first time from a culture of plant tissues contaminated with microbes. The isolated bacteria were tested with four different types of antibiotics. The results showed all bacterial isolates were sensitive to Amikacin, the highest inhibition zone was 21mm against IW31 Bacillus thuringiensis while the lowest was 12 mm against ýIW22 Bacillus ýcereus strain B26, also all isolates were resistant to Ampicillin. Antibacterial assay of extracts amber was found the alcoholic extract of orange amber and the DMSO extract of yellow amber best antimicrobial activity against Enterococcus faecium strain MK748256, Bacillus cereus strain B26 and Bacillus sp. strain PK-7. The yellow amber extract showed wide effectiveness against seven out of eight strains, the highest inhibition value was 20 mm and the lowest was 8 mm, while the orange amber extract was less effective against four strains, the highest inhibition was 15 mm and the lowest was 10 mm. Sterilization of the excised plant parts and the control of microbes is an important stage in the success of plant tissue culture. This study demonstrated the possibility of using natural extracts to reduce bacterial contamination without harming cultured plant tissues.