Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 14, Issue 3, 2012; Page No.(451-456)

EFFECT OF DIFFERENT PARAMETERS ON ALKALINE PHOSPHATASE PRODUCTIVITY FROM A FACULTATIVE ALKALIPHILE BACILLUS FLEXUS FPB17 ISOLATED FROM AN ALKALINE LAKE SEDIMENTS IN INDIA

R. FALGUNI PATEL AND M.C. SHARMA

Abstract

A facultative alkaliphilic bacterium Bacillus flexus FPB17 (GenBank accession no. IN415115), isolated from sediment samples of an alkaline lake, was selected for the production of alkaline phosphatase. Maximum alkaline phosphatase productivity was obtained by using ribose as a carbon source and peptone and meat extract as a nitrogen source in a C:N ratio of 2:1. Alkaline phosphatase production was optimum at pH 9, temperature 35 9C and NaC1 concentration 0.5 g%. The presence of different inorganic and organic phosphate sources (concentration above 0.5 mM) in the medium repressed the enzyme production. Deviations from optimum fermentation parameters drastically disturbed the enzyme production. The alkaline phosphatase productivity was not growth associated and was found to be optimum under aerobic, mesophilic, alkaliphilic conditions. Acephate, the organophosphorous insecticide was degraded by the strain FPB17 in alkaliphilic conditions which indicates the role of alkaline phosphatase enzyme in bioremediation of acephate.

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