Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 14, Issue 3, 2012; Page No.(445-450)

ISOLATION AND ENRICHMENT OF HYDROLYTIC MICROORGANISMS FOR SEWAGE WASTE MANAGMENT: A PILOT STUDY

AMIT SEN, SUMIT KUMAR SINGH AND G. TEJOVATHI

Abstract

Sewage waste and its management is the rising problem in all the growing cities which may be due to the increasing urbanization and industrialization. In the present study, isolation and enrichment of hydrolytic microorganisms was carried out using sewage samples collected from three different locations i.e, Industrial, Residential and Institutional sites of Gwalior city. Physico-chemical characteristics such as Total Dissolved Solids (TDS), Dissolved Oxygen (DO), Chemical Oxygen Demand (COD) and Biochemical Oxygen Demand (BOD), total microbial load were initially analyzed. The pH and DO levels were within the limits in all the three samples tested. However, BOD and COD levels were high indicating the pollution level in the samples. Total bacterial load varied from 35.83 x104 CFU/mL to 781 x104 CFU/mL in the three samples. Least load was recorded in industrial sample while it was high in residential zone. Thirty eight hydrolytic bacterial colonies were isolated by enrichment technique and characterized for their cellulase, amylase and protease enzyme producing activity. Among the 38 isolates, cellulose, amylase and protease enzyme producing colonies were 18.42%, 15.78% and 50%, respectively, while 15.78% colonies were noticed to be producing all the three types of enzymes. Analysis of growth pattern has shown that the application of selected isolates into autoclaved sewage samples has shown the steap increase in the hydrolysis of biopolymers more efficiently as compared to control. Thus application of strains isolation and enrichment of hydrolytic bacteria from same site into sewage may help for the degradation of complex biopolymers present in sewage water and efficiently manage sewage pollution in given complex environmental conditions.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 23, Issue 1, 2021
Vol 22, Issue 4, 2020
Vol 22, Issue 3, 2020
Vol 22, Issue 2, 2020
Vol 22, Issue 1, 2020
Vol 21, Issue 4, 2019
Vol 21, Nov. Suppl. Issue, 2019
Vol 21, Issue 3, 2019
Vol 21, Issue 2, 2019
Vol 21, Issue 1, 2019
Vol 20, Dec. Suppl. No.2, 2018
Vol 20, Issue 4, 2018
Vol 20, Dec. Suppl. Issue, 2018
Vol 20, Oct Suppl. Issue, 2018
Vol 20, Issue 3, 2018
Vol 20, Issue 2, 2018
Vol 20, Issue 1, 2018
Vol 20, Feb. Suppl. Issue, 2018
Vol 19, Issue 4, 2017
Vol 19, Nov. Suppl, Issue 2017
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!