ISOLATION AND ENRICHMENT OF HYDROLYTIC MICROORGANISMS FOR SEWAGE WASTE MANAGMENT: A PILOT STUDYAMIT SEN, SUMIT KUMAR SINGH AND G. TEJOVATHIAbstract Sewage waste and its management is the rising problem in all the growing cities which may be due to the increasing urbanization and industrialization. In the present study, isolation and enrichment of hydrolytic microorganisms was carried out using sewage samples collected from three different locations i.e, Industrial, Residential and Institutional sites of Gwalior city. Physico-chemical characteristics such as Total Dissolved Solids (TDS), Dissolved Oxygen (DO), Chemical Oxygen Demand (COD) and Biochemical Oxygen Demand (BOD), total microbial load were initially analyzed. The pH and DO levels were within the limits in all the three samples tested. However, BOD and COD levels were high indicating the pollution level in the samples. Total bacterial load varied from 35.83 x104 CFU/mL to 781 x104 CFU/mL in the three samples. Least load was recorded in industrial sample while it was high in residential zone. Thirty eight hydrolytic bacterial colonies were isolated by enrichment technique and characterized for their cellulase, amylase and protease enzyme producing activity. Among the 38 isolates, cellulose, amylase and protease enzyme producing colonies were 18.42%, 15.78% and 50%, respectively, while 15.78% colonies were noticed to be producing all the three types of enzymes. Analysis of growth pattern has shown that the application of selected isolates into autoclaved sewage samples has shown the steap increase in the hydrolysis of biopolymers more efficiently as compared to control. Thus application of strains isolation and enrichment of hydrolytic bacteria from same site into sewage may help for the degradation of complex biopolymers present in sewage water and efficiently manage sewage pollution in given complex environmental conditions.
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