DETERMINATION OF ANILINE IN WASTEWATER BY CLOUD POINT EXTRACTION FOLLOWED BY HPLC USING 8- HYDROXYQUINOLINE AS A DERIVATIZATION AGENTGHUFRAN SAAD AHMED, MAHMOOD BASIL MAHMOOD AND BAHAA MALIK ALTAHIRAbstract A procedure, depending on the derivatization and determination of aniline was depicted and validated in this study. 8-hydroxyquinoline (8-HQ) was used as the derivatizing agent for the determination of aniline. An optimization study was performed for the derivatization reaction, i.e., the diazonium coupling reaction, the optimum parameters were as follows: 22 mM of hydrochloric acid, 54mM of sodium hydroxide, and 1.8mM of sodium nitrate. The optimization study of the method of cloud point extraction (CPE) revealed that the extraction solvent was 0.5 ml of Triton X- 100, the optimum temperature was 90 °C, and the incubation time was 25 min. The linearity, correlation coefficients, molar absorptivities, and limits of detection were improved using the CPE method. The extraction product was injected into HPLC. The chromatographic conditions were as follows: the mobile phase was acetonitrile:water 60:40, the stationary phase was C18 RP column, the detection was at visible wavelength of 420 nm, flow rate 1ml/min. The proposed method was applied for the trace detection in environmental application of wastewater. The concentration of aniline in wastewater was in range of 0.2-7 μg/l. |