Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 23, Issue 4, 2021; Page No.(558-562)

ASSESSMENT OF GENETIC DIVERSITY OF FUSARIUM OXYSPORUM F. SP. CICERI USING SSR MARKERS

MINAKSHI PATIL, SATISH KACHARE AND OM GUPTA

Abstract

Fusarium wilt of chickpea caused by Fusarium oxysporum f.sp. ciceri (Foc) is a major constraint to chickpea production world-wide. A total seven different isolates of F. oxysporum f. sp. Ciceri (FOC) were obtained from wilt-infected chickpea plants from different agro climatic regions of central India. The isolates were identified according to the identification key of F. oxysporum. All the isolates were single spored and was stored in tubes containing PDA at 4 °C. All isolates were characterized using four Simple Sequence Repeat Marker (SSR). Cluster analysis revealed that races of FO under study fell in to two groups, major group A and minor group B.Major Group A divided into two sub groups, first subgroup containing two isolates namely I-19 and I-4. Second sub group had also two isolates I-28 and I-1 respectively. Group B divided into two sub group, first sub group containing two isolates I-20 and I-13. Second sub group having only one isolate, i.e. I-80. These diverged from other accessions were placed at end of the cluster.