Ecology, Environment and Conservation Paper

DETERMINATION OF GUAIACOL IN PRESENCE OF MONOVALENT INORGANIC SALT AND ITS ASSESSMENT IN CITRUS SINENSIS LEAVES AND CITRUS LIMON PEELS

N.V.S. Venugopal, N. Swathi and P. Padmavathi

Abstract

Guaiacol (2-methoxy phenol) is a naturally occurring expectorant and biosynthesized by a variety of organisms. Guaiacol is also used medicinally as an expectorant, antiseptic, and local anaesthetic. In this study we reported a method for easy and rapid spectrophotometric determination of Guaiacol in presence of perbromate and periodate. The optimized method has been successfully applied for the determination of Guaiacol in orange (Citrus sinensis) leaves and lemon (Citrus limon) peels. The products obtained shows maximum absorbance at 515 nm in presence of potassium perioadate and 590 nm with potassium perbromate. Beer’s law is obeyed in the linear dynamic range of 20-187.9 g ml-1. The correlation coefficient is 0.996 which indicates the linearity between the two variables. The molar absorptivity coefficient and sandal’s sensitivity of the product are found to be 6.35x104L mol-1 cm-1 and 0.026 g cm-2 respectively. Hence, the proposed method is fairly sensitive and reproducible.