ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF MID GUT MICROBIOTA FROM DIFFERENT ISOLATES OF ANOPHELINE MOSQUITOESAMIT CHATTOPADHYAY, SANTANU MAITRA AND PRANAB KUMAR BANERJEEAbstract Isolation and biochemical characterization of mid-gut microbiota from different isolates of female Anopheles mosquito is performed. Observation reveals that most of these gut dwelling bacteria are Gram negative and also the members of group Enterobacteriaceae.The isolated bacterial colony showed an exponential pattern of growth rate. The aim of this study is to know about the type of heterospecific association between the gut dwelling bacteria and Plasmodium along with the ability of secretion of different types of enzymes viz. DNase, Protease and Amylase. Enzyme assay reveals that the protease activity is more in respect to other enzymes. It indicates that mid- gut microbiota can act as bioremediator.
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