METAGENOMICS TECHNIQUE AS NEW SOURCE FOR ANTIMICROBIAL AGENT PRODUCTIONMohamed T. Shaaban, Reham M. Abdelhamid, Muhammad Zayed and Safaa M. AliAbstract The innovation of antimicrobial agent with elevated effectiveness in conditions agreeable to industrial processes necessities are key to successful development of chemical and drug synthesis. The rate of detection for novel biomolecules using conventional method has been really low down, as the majority of soil microorganisms cannot be cultivated by this means. The objective of the current study was to recognize of separated utilitarian gene(s) as antimicrobial specialist by utilizing metagenomics. A wealthy source of new natural resources for research is soil metagenomes. The process followed by collection of different locations in marine sediment samples from Alexandria and isolation of total DNA, fragmentation by restriction enzymes (HindIII, BamHI, EcoRI and SalI), cloning, expression of the isolated gene(s) in E.coli DH5ï¡. The digested fragments were ligated and cloned into pUC19 vector, then transformed into E.coli DH5ï¡. The 270 resulted clones were screened for plasmid with high copy numbers, only 253 clones contains plasmid. To detect the fragment size of the series of PCR products through using a universal primer (M13). Only the 131 clones which contain fragment (200-3000 bps) were selected for screening of antimicrobial agents. Only 13 clones were positive for antimicrobial agent by using diffusion method. Activity of the antimicrobial agents produced by 13 positive clones were tested towards Bacillus, Escherichia coli and Candida albican, ampicillin and tetracycline (100 mg/ml) were used as standard for comparison. Our data also proved that some of genetically modified clone produce wide spectrum antibiotic and are more effective than ampicillin and tetracycline. |