PHYTOCHEMICAL CHARACTERISTICS AND ANTIMICROBIAL ACTIVITY OF MEDICAL PLANT ZIZYPHUS MAURITIANA AGAINST PSEUDOMONAS FLUORESCENSTitik Dwi Sulistiyawati and R. Adharyan IslamyAbstract The use of medicinal and aromatic plants to treat diseases is vital in the safety of fish communities. The Ziziphus mauritiana show appearance the phenolic compounds that proved had an antimicrobial effect. This study aimed to investigate the phytochemicals composition of Zizyphus mauritiana and its antimicrobial activity against P. fluorescens. Ziziphus mauritiana extraction by maceration methods using methanol solvent. The phytochemical screening was obtained for Alkaloids, Saponins, Triterpenoids, Steroids, and Tannins. The bacterial strain was purchased from Fish Parasites and Diseases, Faculty of Fisheries and Marine Sciences of University of Brawijaya, Malang. Determination of Antibacterial activity using the disc diffusion methods. This study showed the presence of some secondary metabolites such as Alkaloids, Saponins, Triterpenoids, Steroids, Lignin, Phenol, and Tannins. Leaves extract of Ziziphus mauritiana also showed antimicrobial activity against Pseudomonas fluorescens at different concentrations (25, 50, 100, 250, 500 μg/μl and control using antibiotics oxytetracycline of 31,2 μg/μl). The conclusion of this study was the maximum zone of inhibition showed at a concentration of 500 ppm. Antimicrobial activity of Ziziphus mauritiana leaves extract caused by the presence of tannins and alkaloids compounds. |