CHARACTERIZATION OF EXOPOLYSACCHARIDE A PULLULAN PRODUCED BY A NOVEL STRAIN OF AUREOBASIDIUM PULLULANS-SB-1 ISOLATED FROM THE PHYLLOPHANE OF BRASSICA OLERACEA CULTIVATED IN ODISHA STATE, INDIABISHWAMBHAR MISHRA AND SUNEETHA VUPPUAbstract Pullulan, a great natural extracellular polysaccharide is obtained by fermentation using a isolated microbe, Aureobasidium pullulans.-SB-1. The present work reports some of these aspects like screening of a new strain of A. pullulans SB-1 from the phyloplane of Brassica oleracea in natural environment for the first time. The screened microorganism was compared with standard cultures of A. pullulans (NCIM-976, NCIM-1048) for identification and characterization of exo-polysacharide (EPS) production. 2.4% of EPS was produced in the production medium by A.pullulans -SB-1 and its characterization was done by IR-Spectroscopy. The hydrolysis of this EPS was done with the pullulanase enzyme in which maximum 75% of pullulan was hydrolyzed after 300 min yielding 2.80 mg/mL of reducing sugars. The 3,5-dimethylsalicylic acid and Nelson-Somogyi methods were employed in order to estimate the total amount of reducing sugar in the polymer.
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