IN VITRO ANALYSIS OF ANTIOXIDANT ACTIVITY WITH DIFFERENT POLAR SOLVENTS ON CRUDE EXTRACTS OF RHIZOPHORA MUCRONATA AGAINST FREE RADICALS OR DPPH (2,2- DIPHENYL-1-PICRYLHYDRAZYL)Ashari Fahrurrozi, Sri Andayani, Ating Yuniarti and Nelvan SubayuAbstract This study aimed to analyze and determine the antioxidant bioactivity of Rhizhopora mucronata leaf crude extract against free radical scavenging or DPPH (2,2-diphenyl-1-picrylhydrazyl). Antioxidant activity testing was carried out using the DPPH method with a calculation of (%) inhibition for linear regression and inhibition concentration (IC50). The research treatment used 3 different polar solvents, namely methanol, ethanol, and distilled water in the maceration method extract process. Also used is a control in the form of 0.1 mM DPPH solution in p.a. ethanol. Positive control comparisons were treated as samples. R. mucronata extract is known to contain secondary metabolites which have antioxidant properties. The readings using a UV-Vis spectrophotometer showed that the crude extract of R. mucronata with methanol as a solvent had the strongest antioxidant activity with an IC50 value of 8.5744 ppm. While the crude extract with ethanol and distilled water solvent had a value of 29.2815 ppm and 471.4607 ppm, respectively. |