Ecology, Environment and Conservation Paper

Vol 27, Oct Suppl. Issue, 2021; Page No.(226-231)

INVESTIGATION OF ANTIBACTERIAL COMPOUNDS PRESENT IN DIFFERENT SOLVENT EXTRACTS OF AZADIRACHTA INDICA

M. Mukunda Vani, Purushotham Theegala, M.B.Venkata Ramana Reddy, T. Vijaya Kumar

Abstract

In this article, different solvent extracts of Azadirachta indica were tested for antibacterial action. Extraction of Azadirachta indica compounds were done using various solvents like ethanol, methanol, benzene, ethyl acetate and toluene that are frequently used as solvents in industries. The compounds showing antibacterial activity were supposed to be soluble in the above used solvents. In the present study, the antibacterial compound present in the different solvent extracts were compared with the standard drug (streptomycin) which is widely used in pharmaceutical industries. Qualitative analysis of the compounds extracted by a particular solvent was done with Thin Layer Chromatographic (TLC) technique which indicates the existence of different compounds. Statistical analysis (One way ANOVA) was performed and ethyl acetate extract showed a significant antibacterial activity against gram positive bacteria compared to other solvents (viz., ethanol, methanol, benzene and toluene). Similarly benzene extract showed a significant antibacterial activity against gram negative bacteria compared to other solvents (viz., ethanol, methanol, ethyl acetate and toluene).