Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 14, Issue 3, 2012; Page No.(339-345 )

MYCOLOGICAL ASSESSMENT AND EXTRACELLULAR PROTEIN STUDIES OF PATHOGENIC MOLDS IN OYSTER MUSHROOM (PLEUROTUS OSTREATUS) CULTIVATION

Y. SHALINI AND P. PREMASUDHA

Abstract

The Oyster Mushroom of Pleurotus ostreatus is not only the source of nutrients but also pave the way for success of industry, one of the critical point is how to control the pathogenic molds during the process of production. A total of 69 isolates were obtained from mushroom cultivation units in Coimbatore and Erode districts. The fungal isolates were cultured on different media including PDA, SDA, MEA, OMA, WA, GA, CDA, Kcl agar. Growth of the fungal isolates on SDA, OMA and MEA exhibited rapid growth. The effect of pH, temperature, salinity, sources of carbon and nitrogen were tested for different fungal isolates. All the isolates were screened for the extracellular enzymes viz., alpha amylase, alkaline protease, cellulose, xylanase and lipase. Some selected strains that produce high levels of enzymes were grown in shake-flask, quantitatively evaluated for extracellular enzyme production and protein activity. The molecular weight of the enzymes was determined by SDS-PAGE and was found to be ranging from 14.3kDa to 97.4kDa.

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