Ecology, Environment and Conservation Paper

GROWTH MEDIA OPTIMIZATION STRATEGIES TO ACHIEVE HIGHER MYCELIAL AND FRUITING BODY YIELD OF CORDYCEPS MILITARIS FOR LARGE SCALE PRODUCTION

Sourabh Singh Gour, Vivek Kumar Agnihotri, Shilpi Singh, Piyush Kant Rai and Kamlesh Choure

Abstract

Cordyceps militaris, grow in high altitude in Himalayan very harsh conditions it is big challenges for the researchers how to grown under control conditions, this study is based on optimization of maximum mycelial growth of C. militaris. and develop a standard protocol for high yield production of C. militari. The laboratory experiment was carried out to study various factors on the mycelium and fruiting body growth of C. militaris. A vigorous scientific study aimed to identify the various factors like nutrient media, temperature hydrogen ion concentration, and carbon source for the mycelium growth along with grain substrate and liquid medium for the high yield production of C. militaris. The findings revealed that the C. militaris was more likely to develop in CDA (Czapek Dox Agar) nutrient media and maximum mycelium growth were observed at the temperature of 20 °C whereas optimum growth occurred at pH 6. carbon sources such as glucose, and dextrose, at a concentration of 20g/l, enhanced the mycelium growth of C. militaris. further perform the study of fruiting body production in several grains, like brown rice, wheat, millet, chickpea, and maize and finding reveals that maximum fruiting body production found in brown rice along with performing study on different liquid media (M1, M2, M3, M4, and M5) with brown rice as a grain substrate, during observation and analysis conclude that the M3 liquid media producing the maximum yield and primordia and maximum fruiting body of C. militaris. This study parameter provides basic information for vegetative growth and high yield production protocol for C. militaris.