Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 14, Issue 3, 2012; Page No.(319-324)

DEVELOPMENT OF HYDRIDE-GENERATION ATOMIC ABSORPTION SPECTROMETRY FOR SPECIATION OF TRACE ARSENIC IN ENVIRONMENTAL SAMPLES

S.M. TALEBI, M. GHEISARI, S.P. TALEBI AND MD JELAS-HARON

Abstract

A method based on hydride-generation atomic absorption spectrometry equipped with cryogenic collection of hydrides and electrically heated quartz absorption tube was developed for speciation of trace arsenic species in environmental samples. Arsenic species in samples were reduced and converted to different gaseous arsenic compounds by introducing sodium tetrahydroborate solution into the sample. The gaseous arsenic compounds were cryogenically collected in a U-shaped quartz collection tube located in liquid nitrogen. The different compounds were then sequentially released from the collection tube by gradually heating and transferred into the electrically heated quartz absorption tube for determination. The recovery of As(III), As(V), MMA, and DMA by proposed method was investigated through spiking known amounts of each species into the sample. The results indicated that the method is quite precise, sensitive, and reliable for determination of trace arsenic species. Finally the developed method used for speciation of arsenic in environmental samples.

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