Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 14, Issue 3, 2012; Page No.(307-317)

KERATINASE PRODUCTION BY NEW STRAIN OF BACILLUS AMYLOLIQUEFACIENS: APPLICATION OF STATISTICAL EXPERIMENTAL DESIGN FOR OPTIMIZATION OF KERATINASE PRODUCTION

CAROLINA FERRAREZI DA FONSECA, MARIANA CORTEZI, CRISTIAN J BOLNER DE LIMA, REINALDO MARCHETTO, MARCOS MACARI AND JONAS CONTIERO

Abstract

Feathers are rich in amino acids and can be employed as a dietary protein supplement for animal feed. Microbial degradation is an alternative technology for improving the nutritional value of feathers. Other potential applications of keratinase include use in the leather industry, detergents and medicine as well as the pharmaceutical for the treatment of acne, psoriasis and calluses. A new keratinolytic enzyme production bacterium was isolated from a poultry processing plant. To improve keratinase yield, statistically based experimental designs were applied to optimize three significant variables: temperature, substrate concentration (feathers) and agitation speed. Response surface methodology demonstrated an increase in keratinolytic activity at temperature, agitation speed and substrate concentration of 26.69 C, 150 rpm and 2%, respectively. Liquid chromatography revealed the release of amino acids in the Bacillus amyloliquefaciens culture broth, thereby demonstrating the potential of feather meal in the animal feed industry.

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