CHARACTERIZATION AND BIODECOLORIZATION OF AZO DYE USING BACILLUS KOCHII MH152512 FROM TEXTILE DYE EFFLUENT FROM TAMIL NADUP. SAMPATH, M. JEEVANANTHAM, S. ANANDA RAJ, R. FELICIA ROSHINI, U.K. GIRIJA DESIHA, ARUNAVA DAS AND J. BINDHUAbstract The present investigation was focused to decolorize the dye effluent by bacteria. The isolated bacterium was identified as Bacillus kochii MH152512 on the basis of 16S rDNA analysis. Several steps were initiated in treating the dye effluents and the degradation activity of these effluents by B.kochii was determined by the physical and chemical property of the effluent. This strain was identified to show maximum decolorization as compared to other bacterial strains isolated from the effluent. The B. kochii strain was investigated for various parameters such as effect of pH, temperature along with carbon and nitrogen sources as supplement nutrients. The concentration of the dye degraded activity of the effluent was confirmed using UV- spectrophotometer, which is recorded continuously for 3 days. Complete decolorization was observed at pH 6 and at temperature 37 °C. Phytotoxicity studies revealed that there is a reduction in toxicity of the decolorized dye by bacteria. Also cleavage of bond after decolorization was confirmed using FTIR. Then the medium optimization was done through Response Surface Methodology, in which optimum pH was obtained at 6 and temperature at 37°C and then the model was predicted through Box-Behnken method. |