EFFECT OF PRESERVATIVES AND PESTICIDES ON MITOTIC INDEX OF ALLIUM CEPA ROOTS -BIOLOGICAL MODEL EXPERIMENT FOR GENOTOXICITYJYOTCHNA GOGOI, KARABI DAS AND PAPIA DUTTA
In research, Genotoxicity model: Allium cepa has often been used due to its cost effectiveness and basic laboratory requirements. Many studies based on mitotic index in Allium cepa roots have been performed to observe toxicity of various contaminants. So, a study was designed to conduct genotoxic evaluation in Allium cepa roots caused by food preservatives and pesticides which could be used in college experiments with minimum health concern. Microscopic observation like Chromosomal aberrations and mitotic index was documented by Foldscope. Allium cepa bulbs were allowed to grow under different treatments- normal distilled water (treatment 1), malathion (treatment 2), chlorpyrifos (treatment 3), residual pesticides (treatment 4), monosodium glutamate (treatment 5), sodium benzoate (treatment 6) and propyl gallate (treatment 7) with an optimal dose concentration (100 Î¼g/ml) with an interval of 24 hrs. Mitotic index of the dividing cells were calculated for duration of 24 hrs to 72 hrs. The results showed highest mitotic index in normal (treatment 1): 63Â±0.8 and 68Â±1.1, whereas treatment 5 showed lowest mitotic index of 22Â±0.46 and 15Â±1.02 in 24 hrs and 72 hrs, respectively. Thus, it could be concluded that food preservatives Monosodium glutamate can be used as standard for the experiment in college which would help the students to understand genotoxicity at ease with minimum use of sophisticated instruments.