Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 23, Issue 3, 2021; Page No.(339-346)

REPORTS ON DIRECT AND INDIRECT ORGANOGENESIS THROUGH TISSUE CULTURE IN CITRUS

PRIYANKA SHARMA BIDHAN ROY AND MONISH ROY

Abstract

Citrus is considered as one of the most essential fruit crop of the world due to its high nutritional. value as well as its great production potential; several species of citrus are cultivated in most tropical and subtropical regions of the world. Genus citrus includes more than 162 species which belong to the family Rutaceae and sub family Aurantoideae. Commercially important citrus species includes Kumquat (Citrus japonica), Sweet orange (C. sinensis L. Osbeck), Grapefruit (C. paradise Macf.), Pummelo (C. grandis Osbeck), Acid Lime (C. aurantifolia Christm) and Lemon (C. limon (L) Burn F.) etc. Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. This technique involves callus induction from explants, morphogenesis, shoot development and finally root development to regenerate into a complete somaclone. Production of plants in sterile containers that allows them to be moved with greatly reduced chances of transmitting diseases, pests, and pathogens. Hence, application of tissue culture biotechnology in the field of agriculture seems very crucial so as to increase agricultural productions including citrus for the purposes of feeding the population with no need of international aids. Development of protocols for successful production of callus initiation system through tissue culture for further regeneration of shoots and roots in several species of Citrus have been reviewed in this paper.