BIOCONVERSION OF SYNGAS TO BIOETHANOL USING ACETOGEN UNDER SUBMERGED FERMENTATIONSWAYAMSIDHA PATI, MAHENDRA K. MOHANTY, SUDIPTA MAITY, SWATI MOHAPATRA AND DEVI P. SAMANTARAYAbstract Crude oil extinction and booming energy consumption coupled with climate crisis to pave the way of academia & industry for development of a sustainable biofuel. Hence, economic & eco-friendly bioethanol comes into limelight which is a superior substitute of gasoline used in transportation sector as it doesnât contribute noxious gases to global warming. This research deals with synthesis of bioethanol through Syngas fermentation using acetogens. Therefore, in toto 07 acetogens were isolated from compost sample in ATCC medium no. 1754 under anaerobic condition. Among them, the acetogen S1 a has the ability to produce bioethanol as confirmed from pyridinium chlorochromate assay. The screened acetogen produced 1.44% of bioethanol through syngas (60% CO+ 20% CO2+ 15% H2+ 5% CH4) fermentation carried out at 37 oC for 72 hours in aspecially designed culture bottle. The bioethanol production and its concentration was confirmed by GC analysis. The bioethanol producing acetogen was identified as Clostridium sp. S1a by API 20A system. Further, bioethanol production under optimized condition and molecular characterization of the Acetogen is highly essential in this regard. |