MICROPROPAGATION STUDIES IN ASHWAGANDHA (WITHANIA SOMNIFERA)S. G. NAWSUPE AND S.C. PATILAbstract The present investigation on micropropagation studied in ashwagandha (Withania somnifera) were to study the response of various genotypes on different media. The various genotypes were collected from Medicinal and Aromatic Plant Project, MPKV, Rahuri, Dist, Ahmednagar. Among the different media concentration tried for the establishment of the culture. Least period for the establishment for all genotypes responded on MS media supplemented with 2 mg/l BAP and 0.4 mg/l KIN. However embryogenic callus was formed on MS medium containing 2,4-D (0.5 mg/l) after 2 weeks of subculture. Regeneration was noticed within 3.66 to 12.33 days. The genotype RAS-2 took minimum days 3.66 for emergence of first shoots on media MS + 2 mg/l BAP+2 mg/l KIN. Maximum number of multiple shoots (7.00) were produced by RAS-2 genotype on media MS+3mg/l BAP+1mg/l IAA). The optimums rooting to the shoot were recorded on MS supplemented with 1 mg/l IBA. Maximum percentage of survival 80% observed in genotype RAS-2 plantlets was established after hardening. |