Ecology, Environment and Conservation Paper

BIOAUTOGRAPHY OF ETHANOL EXTRACT FROM CARICA PAPAYA LEAVES FOR ANTIMICROBIAL ACTIVITY AGAINST STAPHYLOCOCCUS AUREUS, E. COLI AND BACILLUS SUBTILLIS

Tri Puji Lestari Sudarwati, Galuh Gondo Kusumo, M.A. Hanny Ferry Fernanda and A. Soegianto

Abstract

In the present study, we explore the use of papaya leaves ethanol extract as an antibacterial using bioautography method. Bioautography of papaya leaf ethanol extract against Staphylococcus aureus, E. coli and Bacillus subtillis with n-butanol, acetic acid, distilled water with the ratio 4: 1: 5 yielded the dissolved compound of tannin. Furthermore, the tannins contained in papaya leaves has moderate antibacterialNroperties.