REDESCRIPTION OF STICHOPUS MONOTUBERCULATUS (ECHINODERMATA, HOLOTHUROIDEA, STICHOPODIDAE) OF PARANG ISLAND, KARIMUNJAWA ARCHIPELAGO, CENTRAL JAVA, INDONESIAWidianingsih Widianingsih, Retno Hartati, Hadi Endrawati, Robertus T. Mahendrajaya and Agoes SoegiantoAbstract Stichopus monotuberculatus (Quoy & Gaimard, 1833) belongs to family Stichopodidae, has high commercial value in fisheries trade world. Most of the sea cucumbers belong to family Stichopodidae have long been used for nutritional food and medicine. The objective of the study was to redescribe this species by its phenotype characteristics through morphological keys and review of their ossicles as well as genotype by DNA mapping. The samples were collected from Parang Island and preserved in alcohol 96%. Commercial bleach were used to remove tissue and take the ossicles, then they were examined under light microscope. For genotype characteristic (DNA mapping) extraction DNEasy of tissue (qiagen) was used for extraction of DNA. Primer CO1 was used to amplify the genom. Samples showed that morphologically they were grey-green colour with numerous small dark patches ventrally and grey green to orange-brown with dark green to black patches dorsally with square thick integument/body wall. Calcareous ring radial pieces had a posterior notch and four short anterior points whereas the interradial pieces had a long anterior tooth. The ossicles showed numerous tables-shaped ossicles in the anterior and dorsal tissue of the bodywall, but norossete-shape in dorsal body wall which is the characteristic of S. monotuberculatus. DNA sequencing of nine samples showed that all samples had got 93-99% similaritywith Stichopus monotuberculatus haplotype4, 5, 9, and 13. This result confirmed the identification through morphology and ossicles characters. It is approved the presence of S. monotuberculatu (Quoy and Gaimard, 1833) in Parang Island, Karimunjawa Archipelago, Jepara. |