THE EFFECT OF FIG LEAVES (FICUS CARICA) ETHANOL EXTRACT ON TOTAL OF SPERMATOGENIC AND LEYDIG CELLS OF MALE MICE (MES MUSCULUS) EXPOSED WITH LEAD ACETATEFRISTI SINTYA HERAWATI, SRI AGUS SUDJARWO, TJUK IMAM RESTIADI, WIDJIATI, LITA RAKHMA YUSTINASARI AND ERMA SAFITRIAbstract This study aims to prove the effect of Tin leaves (Ficus carica) ethanol extract on the number of spermatogenic cells and the number of Leydig cells of mice exposed with lead acetate. There were 24 male mices as samples and divided into 4 groups. Group C- is a group that was given aquadest for 35 days, group C+ was given lead acetate 50 mg/kg BW for 21 days, groups P1 and P2 were given Tin leaves ethanol extract at a dose of P1 (166.4 mg/kg BW) and P2 (dose 332, 8 mg/kg BW) for 35 days. After one hour both groups were given lead acetate at a dose of 50 mg/kg BW on days 14-35. The result showed a significant difference (p< 0.05) on spermatogonia cells from C+ group (36.20a (+/-) 2.29), P1 (44.97b (+/-) 2.55), C- (48.57bc (+/-) 4.96) and P2 (51.53c (+/-) 2.94); on primary spermatocytes C+ group (34.23a (+/-) 3.96), P1 (47.60b (+/-) 8.94), P2 (55.63c (+/-) 4.82), and C- (69.03d (+/-) 4.22); whereas for spermatids C+ group (44.43a (+/-) 3.79), P1 (60.83b (+/-) 7.29), and C- (77.73c (+/-) 5.58 ); then Leydig cells from C+ group (23.70a (+/-) 4.60), C- (31.80b (+/-) 5.29), and P1 (28.30ab (+/-) 3.21). This study concluded that dose of 332.8 mg/kg BW was an effective dose to protect spermatogenic cells and Leydig cells against lead acetate exposure. |