THE EFFECT OF L-ARGININE TO THE NUMBER OF SERTOLI CELLS AND LEYDIG CELLS OF MICE (MUS MUSCULUS) AFTER EXPOSURE TO HIGH TEMPERATUREK.K. BARIYAH, E.B. AKSONO, L.R. YUSTINASARI, S. SUSILOWATI AND WIDJIATI AND ARIMBIAbstract The purpose of this study was to find out the effect of L-arginine on the number of Sertoli cells and Leydig cells in mice (Mus muculus) after exposure to high temperature. The subjects of this study were 30 male mices, 8 weeks old. This research was conducted by using Randomized Block Design experimental group 2x3 factorial design with 5 replications. The negative control group was given aquabidest without heat stress, while treatments group 1 and 2 were given L-arginine at a dose 1.3 mg/kg BW and 2.6 mg/kg BW. The positive control group was given aquabidest with high temperature exposure at 40 oC, while treatment groups 3 and 4 were given high temperature exposure at 40 oC with L-arginine at a dose 1.3 mg/kg BW and 2.6 mg/kg BW. Sertoli cells and Leydig cells were calculated and analyzed using two way ANOVA followed by Duncan Test. The results of this study were revealed that the treatment of heat temperature can reduce the number of Sertoli cells and Leydig cells in mice (p^0.05). The treatment with L-arginine orally to mice can increase the number of Sertoli cells with a dose 1.3 mg and Leydig cells with a dose 2.6 mg (p^0.05). This study concluded that there is no interaction between the heating temperature and the doses of L-arginine to the number of Sertoli cells and Leydig cells in mice (p^0.05). |