CONCENTRATION AND ACTIVITY OF CHOLINESTERASE IN COWS PLASMA AS AFFECTED BY ORGANOPHOSPHATE PESTICIDESALI HAROKAN, TOTONG KAMALUDDIN, DANIEL SAPUTRA AND NURHAYATI DAMIRIAbstract Unhealthy environment such as landfills, will result in the exposure of livestock grazing there, and affect the growth and development of these animals. The activity of blood cholinesterase enzymes in animals is used to diagnose and monitor poisoning caused by organophosphate pesticides. Quantitative research with a survey design using a cross sectional design using 35 samples of cows (wild). The study was conducted in January - June 2020. Measurement of blood plasma cholinesterase enzyme activity concentration using the A15 biosystem tool DGKC-Colorimetric Kinetic method. This study aims to evaluate the level of the cholinesterase enzyme as a marker of organophosphate poisoning in cows. Blood samples were taken from a ventrolateral vein of a cows neck, male and female, aged 1-5 years collected from local cows in the landfill. The results showed that cows that were farmed in the land fills (TPA) were exposed to organophosphate pesticides as indicated by a decrese in the concentration and activity of the cholinesterase enzyme in the cows plasma. Cholinestrase enzyme levels in male cows was 0.173 (+/-) 0.081 U/mL and in female cows was 0.188 (+/-) 0.071 U/ml. The highest concentration of cholinesterase enztme activity was at 1.5 years old cows that was 0.22 (+/-) 0.042 U/ml and the lowest was at 3 years old cows that was 0.14 (+/-) 0.078 U/ml. |