Ecology, Environment and Conservation Paper

Vol 27, Issue 1, 2021; Page No.(282-287)

ISOLATION AND CHARACTERIZATION OF SULFATE REDUCING BACTERIA FROM CULTIVATION POND SEDIMENTS

Bakhtiar Ibrahim, Sukoso, Rudi Sulistiyono and Saida

Abstract

Sulfate reducing bacteria (SRB) are bacteria that play a role in the bioremediation process. These bacteria are commonly found in anoxic environment, especially at the bottom of the sediments. This study aims to isolate and characterize sulfate reducing bacteria from cultivating pond sediments. A total of 20 bacterial samples were isolated from pond ecosystems in the Kuri area of Maros Regency, South Sulawesi, Indonesia. Growth of sulfate reducing bacteria was conducted using selective media postgate B by observing the change in color of the media to black. Purification is done by observing the colonies that grow in the media Nutrient Agar (NA) after incubation for 24 hours. Characterization tests include observations of colony morphology and bacterial cells, as well as biochemical tests. The results showed that SRB has four best isolates in sequence, 3A, 3B, 3D, and 4D. Based on the results of the characterization test using profile matching isolate 3A, it was identified as a member of genus Desolfobulbus.

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