MICROPROPAGATION OF THERAPEUTICALLY IMPORTANT SWARNA JIBANTI (COELOGYNE CRISTATA LINDL.) THROUGH PSEUDOBULB SEGMENTS-A STUDY IN VITROVishal SharmaAbstract Presently, an efficient protocol for micropropagation using the pseudobulb derived from in vivo plants of Coelogyne cristata Lindl was developed. The regeneration competence of the pseudobulb segments was markedly influenced by physiological age of the mother plant, position on donor axis and the nutrient regime. Juvenility of the tissues emerged as the important factor in inducing cell proliferations, as the explants from well developed pseudobulb remained recalcitrant to regeneration, whereas regeneration in the freshly formed pseudobulbs was obligatory to the exogenous application of cytokinin in dose double to auxin (10.25±0.25), which multiplied through budding. The pure-line stability of regenerants was confirmed at chromosome level (2n=38). The well rooted regenerated plantlets were acclimatized successfully in the potting mixture containing moss, pinebark, brick pieces (5-7mm size) and charcoal pieces (1:1:1:1) mixture with 90% survival success. |