Pollution Research Paper

Vol 39, Issue 4, 2020; Page No.(1262-1265)

ANTIBIOTIC RESISTANCE PROFILE OF ESCHERICHIA COLI ISOLATED FROM BEEF SOLD AT SEVERAL WET MARKET IN SIDOARJO, INDONESIA

P. B. PRATIWI, S. HIDANAH, M. H. EFFENDI, A. T. S. ESTOEPANGESTIE, D. RAHARDJO AND WIWIEK TYASNINGSIH

Abstract

The purpose of this study was to know antibiotic profile of Escherichia coli to several antibiotics. Total of 30 swab samples of beef sold at five different wet markets in Sidoarjo, were purchased from six beef vendors of each wet market. Isolation and Identification of E. coli was performed by bacteriological method using BGBB, EMBA, TSIA, and SIM. The antibiotic susceptibility test was done according to Kirby-Baurer method using five antibiotic disks, includes Ceftriaxone 30 μg, Erythromycine 15, Gentamycin 10, Meropenem 10, and Tetracycline 30. The antibiotic resistance profile of E. coli showed resistance to Tetracycline 31.5% (6 isolates) and each 5.2% (1 isolates) to Ceftriaxone and Gentamycin, intermediate to 5.2% antibiotics Erythromycine, Gentamycin, and Tetracycline and Susceptible 100% to Meropenem, 94.7% to Ceftriaxon, 89.4% to Gentamycin and 63.1% to Erythromycine antibiotics, and 10.5% to Tertacycline antibiotics.