Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol.14, Issue 1, 2012; Page No.(83-86)

BIO EFFICACY OF AQUEOUS RHIZOME EXTRACT OF KYLLINGA NEMORALIS (FORSTER) DANDY AND TRICHODERMA VIRIDE PERS. FR AGAINST SEED BORNE FUNGI OF OKRA

P.M. BEEBI RAZEENA and RASHEED AHMAD

Abstract

The present investigation was undertaken to study the bio efficacy of aqueous rhizome extract of Kyllinga nemoralis. Common weed of Kerala and Trichoderma viride, against seed borne mycoflora of okra (Abelmoschus esculentus (L) moench) cultivated in Malabar, Kerala, India. Seeds of both local and hybrid (salkeerthi) varieties collected during rainy and summer season of 2004 and 2005. Invitro cullture studies of Kyllinga nemoralis rhizome extract showed 97.7% inhibition of Aspergillus flavas, 94.4% inhibition of Apergillus niger and Fusarium moniliforme and 88.8% inhibition of Macrophomina phaseolina, were the four selected dominant pathogenic fungi with a corresponding increase in efficiency in treated seeds under in vitro conditions. The treated seeds also showed an increase in germination (97%) where as under conditions in vitro Trichoderma viride showed inhibition of Fusarium moniliforme and Macrophomina phaseolina (100%) and Aspergillus flavus and Aspergillus niger (83.3%). Germination efficacy of treated seeds invitro showed 83.7%. In both cases an enhanced vigour index was observed. Use of the above mentioned bioagent showed a drastic reduction of tested pathogenic fungi.

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