PCR BASED DETECTION OF COLLETOTRICHUM GLOEOSPORIOIDES (PENZ.) PENZ. AND SACC. THE CAUSATIVE AGENT OF LEAF SPOT DISEASE OF MURRAYA KOENIGII L.Midhila Padman and G. R. Janardhana
The curry leaf tree is vulnerable to leaf spot disease characterized by black necrotic spots on leaves. Not much work has been done on leaf spot of Murraya koenigii. Hence this research work has emphasized in elucidating the disease causing agent and determining their role in producing the observed symptoms. Isolates of Colletotrichum spp. from infected curry leaf were characterized by molecular methods and compared with morphological identification. The two its universal primer pair used for PCR 5- TCC GTA GGT GAA CCT GCG G- 3 and 5 - TCC TCC GCT TAT TGA TAT GC- 3 determined the causative agent as Colletotrichum gloeosporoides.
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