EFFECTS OF 2,4-DICHLOROPHENOXY ACETIC ACID AND 6- BENZYLAMINOPURINE ON CALLUS INDUCTION AND SECONDARY METABOLITES OF ELEPHANTOPUS SCABER L.Junairiah, Fransisca Dewi Maya and Edy Setiti Wida Utami
This study was aimed to investigate the effect of growth regulators combination 2,4-dichlorophenoxy acetic acid (2,4-D) and 6-benzylaminopurine (BAP) on induction time, percentage, fresh weight, dry weight, morphology, and secondary metabolites profile of callus from Elephantopus scaber L. leaves. This study was an experimental laboratory study with a completely randomaized design (9 treatments combined concentration with each 3 replications, there was D0.0B0.0(control); D0.5B1.5; D0.5B2.0; D0.5B2.5; D1.0B0.5; D1.0B1.5; D1.0B2.0; D1.0B2.5 and D1.5B1.5). E. scaber L. leaf explants were cultured on solid Murashige and Skoog (MS) medium supplemented with growth regulators 2,4-D and BAP. Qualitative data were obtained from leaf callus morphology and analysis of secondary metabolites profile from callus and leaf extract of E. scaber L. Quantitative data were obtained from a percentage of callus formed by explant, observation time of callus induction, callus fresh weight, and callus dry weight, then it were statically analyzed. The result of this study showed 2,4-D and BAP had effect explant growth on leaf of E. scaber L. Combination of concentration 0.5 mg/L 2,4-D and 2.0 mg/L BAP (D0.5B2.0) showed the fastest induction at that 5.67 days. D0.5B1.5 treatment produced the highest of fresh weight at 0.3176 grams, meanwhile D1.0B1.5 treatment produced the highest of dry weight at 0.0440 g. Callus morphology in D0.5B2.5 and D1.0B2.5 treatments had friable texture, while the other treatments had compact texture, and commonly callus of E. scaber L. has light green colors. The compound of secondary metabolites in the callus and leaf extract of E. scaber L. was very diverse including flavonoid, alkaloid, terpenoid, and saponin.