STUDY OF IMMUNOMODULATORY ACTIVITY OF ELECTROAACUPUNCTURE TECHNOLOGY AND HYLOCEREUS UNDATUS FRUIT EXTRACT IN RATSMochammad Juddy Widjaja, Agus Sjafarjanto, Miranti Candrarisna, Arif Nur Muhammad Ansori, Suhailah Hayaza, Raden Joko Kuncoroningrat Susilo and Saikhu Akhmad Husen
The purpose of the present study was to describe the mechanism of electroacupuncture technology and standardized fruit extract of Hylocereus undatus as an immunomodulator in rats. It was an experimental study with a post-test only control group design. Samples consisted of 30 to male white rats aged 5-6 months and weighed 250 grams, which were divided into five treatment groups and 6 replications in every group. An electroacupuncture technology was applied the acupuncture points of Hegu (Li 4), Zu San Li (ST.36), and Sanyinjiao (L.6) in order to enhance cellular and humoral immune responses. In vitro tests of immunomodulatory activity were conducted in 4 stages: isolation of macrophages, isolation of lymphocytes and macrophage activity test, blast transformation test with MTT assay, standardization extract of Hylocereus undatus fruit with the TLC method and identification of Hylocereus undatus conducted at the Purwodadi Botanical Garden, Pasuruan, Indonesia. The data was analyzed by using ANOVA. We showed that Hylocereus undatus fruit extract combined with the electroacupuncture technology was capable of enhancing and activating phagocytic activity of macrophages in the cellular immune responses and activating B lymphocytes in the humoral immune responses to the antigen of canine parvovirus (CPV) and increasing the levels of lymphocytes, monocytes, erythrocytes, and hemoglobin of the blood feature of the rats infected with CPV antigen.