Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol.14, Issue 1, 2012; Page No.( 33-38)

ISOLATION, CHARACTERIZATION AND PURIFICATION OF CHITINASE FROM PENICILLIUM, PSEUDOMONAS AND BACILLUS AMYLOLIQUEFACIENS

V.S. Sarvani and Priya R. Iyer

Abstract

Chitin degradation can be initiated by chitinases to oligo N-acetyl glucosamine (NAG) chains. The oligomers, tri-NAG and di-NAG are subsequently metabolized to NAG monomes. The monosaccharides thus released can then be metabolized by many organisms. Chitinases acts as a good insecticide and fungicide. The organisms producing Chitinase were isolated from soil. The isolated organisms were identified to be Penicillium chrysogemum, Peseudomonas spp. and Bacillus amyloliquefaciens and grown on chitin minimal media for the enzyme production which was assayed by Aminoff et al. 1952, method of colorimetry. The enzyem was then precipitated by ammonium sulphate precipitation. SDS-PAGE. Optimization of conditions for enzyme production enzyme and application for anti - fungal activity was studied.

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