Ecology, Environment and Conservation Paper

Vol 26, Issue 4, 2020; Page No.(1727-1730)

POTENCY OF PHOSPHATE SOLUBILIZING YEAST FROM MANGROVE CENTER IN JENU, TUBAN, INDONESIA

Tri Nurhariyati, Puspita Ayu Dewi, Eva Watamtin Widhiya, Erna Kristiana Dewi, Tini Sutiningsih and Fatimah

Abstract

The purpose of this research was to isolate potential phosphate solubilizing yeasts from rhizosphere soil in Mangrove Center Jenu Tuban, Indonesia. Soil samples were collected using purposive sampling technique. Yeast were isolated from the soil and purified using YMEA (Yeast Malt Extract Agar) medium. These isolates were inoculated in Pikovskaya Agar medium using spot inoculation method, and incubated for 3 days to screen for the phosphate solubilizing activity. The positive result was indicated by formation of clear zone (halo zone) surrounding growing colonies. Seventeen yeasts indicated the potency of phosphate solubilizer. Isolates with the highest potential were K14, K1, and K8 with Phospate Solubilizing Indexes (PSI) 2.58, 2.55, and 2.48, respectively. K14 was identified as the member of genus Candida.