Ecology, Environment and Conservation Paper

STUDY OF THE ANTIBACTERIAL SENSITIVITY OF STAPHYLOCOCCUS AUREUS ISOLATED FROM DAIRY CATTLE

Gulzhagan Chuzhebaeva, Bakhit Baimenov, Gulnur Aliyeva, Rita Siugzdiniene, Anara Mendybayeva and Raushan Rychshanova

Abstract

The aim of this work was to isolate and to identify Staphylococcus aureus from dairy cattle in the northern region of Kazakhstan and to determine phenotypic and genotypic resistance profiles of isolated strains. During 2018-2019, 1245 samples of cow milk were examined, from which 68 strains of Staphylococcus aureus were isolated and confirmed by detection of species-specific thermonuclease gene. The most common genes encoding antimicrobial resistance were as follows: blaZ was detected in 29 isolates; aac(6)-aph2 in 5 isolates, aph(3) in 6 isolates ermC in 14 isolates, ermB - in 19 isolates, tetK gene in a single isolate and tetM gene in 2 isolates from 68 isolates tested. Phenotypical resistance to cefoxitin demonstrated high rate (28 isolates out of 68) of methicillin-resistant S. aureus however, these isolates did not harboured neither mecA nor mecC genes. According to the data obtained it may be presumed that methicillin-resistant S. aureus prevalent in cattle population in the Northern Region of Kazakhstan carries unknown resistance determinant encoding resistance to beta-lactam antibiotics.