Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 22, Issue 4, 2020; Page No.(648-653)

ISOLATION AND CHARACTERIZATION OF BACTERIA WITH BIOCHEMICAL IMPORTANCE FROM SOIL SAMPLES OF RANCHI CITY, INDIA

SUNIL KUMAR JHA

Abstract

In the present study soil samples from Ranchi municipality area was used for isolation and characterization of bacteria having biochemical potential and pharmacological importance. Total twenty five soil samples were collected from fish, vegetables and fruits dump area from Ranchi town. Bacterial population was sub-cultured in Trypticase Soya Agar (TSA) Plate. Fifteen colonies were isolated, cultured and characterized by Gram staining and Biochemical tests. Five isolates were found to be gram negative while ten were gram positive. All isolates were positive in oxidase, catalase, citrate, and protease tests. Eight isolates showed coagulase negative and seven were coagulase positive. The intrinsic levels of resistance to antibiotics Chloramphenicol, Kanamycin, Streptomycin, Ampicillin, Tetracycline and Nalidixic acid were studied and resistance pattern showed, all the bacterial isolate were found to be resistant to Kanamycin, Streptomycin and Ampicillin till the concentration of 200 μg/mL. of the three isolate, showed maximum resistance 200 μg/mL to all the antibiotics tested, three isolate could grow only up to 50 μg/mL in Chloramphenicol. The two can be further differentiated from each other in their resistance to Nalidixic acid and Tetracycline. It was found that all bacterial isolates were sensitive to Tetracycline, Chloramphenicol, Gentamycin, Ciprofloxacin, Azithromycin and Ceftriaxone.

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