THE BIOSURFACTANT ACTIVITY OF SUPERNATANT FERMENTATION BROTH ISOLATES BACTERIAL ORIGIN OF SURABAYA’S WONOREJO MANGROVE SEDIMENT AND ITS POTENTIAL AS AN ANTIFUNGAL AGAINST CANDIDA ALBICANS ATCC 10231Bima Widya Pramudianto, Suryanie Sarudji, Rahmi Sugihartuti, Didik Handijanto, Wiwiek Tyasningsih and Eduardus Bimo Aksono
The purpose of this research was to know genus, biosurfactant activity and antifungal activity potency of bacterial isolate from mangrove sediments of Wonorejo Surabaya. Mangrove Wonorejo Surabaya is known as contaminated of heavy metals and hydrocarbon. Heavy metals and hydrocarbon contamination could induce microorganism to produce biosurfactants. Biosurfactant activity test used oil spreading, drops collapse, and parafilm test and antifungal activity test used agar well diffusion method. The result of this research showed that Genus of bacterial isolate belong to Bacillus because the bacterial isolate was Gram positive, bacil shape, aerob, and having endospore with ellipse shape which was in center of the cell. The result of biosurfactant activity with oil spreading method showed by clear zone. The average clear zone was 48.88 mm, the average droplets of parafilm test is 6.41 mm, and the result drops collapse test is positive showed by spread and drowned cell free supernatant of bacterial isolate. Cell free supernatant of bacterial isolate could inhibit growth of Candida albicans. The average inhibitory diameter is 31.25 mm. The conclusion of this research proved that bacterial isolate belong to Genus Bacillus which have biosurfactant and antifungal activity of Candida albicans.