Ecology, Environment and Conservation Paper

ISOLATION AND IDENTIFICATION OF LACTOBACILLUS SP. BACTERIA IN ASIAN PALM CIVET (PARADOXURUS HERMAPHRODITUS) FECES

Dinda Jelita Jauharah, Sri Chusniati, Mohammad Anam Al Arif, Wiwiek Tyasningsih, Suryanie Sarudji, Agnes Theresia and Soelih Estoepangestie

Abstract

The purpose of this research was to determine the presence of Lactobacillus sp. bacteria in Asian palm civet (Paradoxurus hermaphroditus) feces. This study totally used four samples of civet feces that obtained from civet farms at Cikole Lawang, Malang. The bacteriological isolation and identification of Lactobacillus sp. was determined by inoculation on pre-enrichment using de Man Rogosa Sharpe (MRS) broth and enrichment media using MRS Agar, followed by biochemically in Gram stain, Native isolate, Catalase test, Sulfide Indol Motility (SIM), Triple Sugar Iron Agar (TSIA), Methyl Red Voges Proskauer (MRVP), and Sugar fermentation test. Gram staining used to distinguish and classify bacterial species into two groups which is gram positive or gram negative and the result said that the bacteria has rods shape and including into Gram positive group. MRVP, TSIA, and sugar fermentation test are used to know the characteristic and fermentation ability of the bacteria. The results of this study showed that Lactobacillus sp. detected in all samples but in each samples has a different sugar fermentation abilities, one of the four samples has a motile character. Lactobacillus bacteria that grows may have different species.