Ecology, Environment and Conservation Paper

Vol 26, Nov Suppl. Issue, 2020; Page No.(261-264)

ANTIBIOTIC RESISTANCE PROFILE OF ESCHERICHIA COLI ISOLATES COLLECTED FROM CLOACA SWABS ON LAYING HENS IN UDANAWU SUB-DISTRICT, BLITAR DISTRICT, INDONESIA

Freshinta Jellia Wibisono, Bambang Sumiarto, Tri Untari1, Mustofa Helmi Effendi, Dian Ayu Permatasari and Adiana Mutamsari Witaningrum

Abstract

The use of antibiotics in poultry can encourage antibiotic resistance, one of which is the bacterium Escherichia coli. This research was carried out in a cross-sectional descriptive, aimed at finding out the resistance profile of Escherichia coli bacteria on laying hens in Udanawu sub-district, Blitar district. Samples of 30 cloaca swabs were taken from the layer chicken. Positive isolates of Escherichia coli were tested for antibiotic sensitivity on Mueller-Hinton agar. The results showed that there were 90% positive Escherichia coli on laying hens in Udanawu sub-district, Blitar district. The highest percentage of antibiotic resistance in Escherichia coli was 96.3% in the erythromycin antibiotic, 77.78% in ampicillin, 66.67% in streptomycin, 59.26% in sulfamethoxazole-trimethoprim, and 44.44% in tetracycline. Escherichia coli which has multidrug resistance properties on laying hens in Udanawu sub-district is quite high, amounting to 70.37%. The overall resistance in Escherichia coli to some antibiotics shows that the resistance on laying hens is relatively high, so that the supervision of antibiotic use in animal husbandry needs to be increased as an effort to prevent broader antibiotic resistance.