ISOLATION OF ACTINOMYCETES FROM MANGROVE SEDIMENTS AT UJUNG PANGKAH, GRESIK, INDONESIAA.R. Hidayatullah, R. Sugihartuti, D. Handijatno, S. Chusniati, L. Maslachah and S. SarudjiAbstract This research was conducted to get actinomycetes isolate from mangrove sediments at Ujung Pangkah in Gresik-Indonesia. Sediment samples were taken from mangrove rhizosphere in Ujung Pangkah on 3 different location sampling areas. Ten grams of soil sample was accurately weighed and transferred to add 100 mL of sterile NaCl 0.9% mixed well for 10 minutes. The resultant solution was serially diluted up to 10 -12 (10 -3, 10 -4 until 10 -12) with NaCl 0.9%. One millilitre of each intermediate dilution (10 -3, 10 -4 until 10 -12) was added to 10 mL of sterile molten Starch Casein Agar medium which has been supplemented using both chloramphenicol and griseofulvin each one 0.05 ppm individually in separate flasks. The plates were incubated for the growth of actinomycetes colonies at 28 oC and observed intermittently during incubation. After 7 days of incubation, the colonies showing the characteristics of actinomycetes (rough, chalky, powdery appearance radiating growth and leathery texture) were observed. Identification of actinomycetes isolates was conducted by observing macroscopic characteristic of the colony, microscopic of conidial, bacterial cell and the ability of bacteria to resist from acid alcohol. Identification was done based on Bergeyâs manual of determinative bacteriology. The result of this research obtained 9 different isolates actinomycetes. The 9 isolate was identified as 3 genera from actinomycetes. The genera of isolate obtained are Micromonospora, Nocardia and Streptomyces. |