Ecology, Environment and Conservation Paper

EFFECT OF ADDITION OF ONION (ALLIUM CEPA L.) EXTRACT IN RINGER’S DILUENT ON SPERMATOZOA QUALITY OF GALLUS DOMESTICUS AT ROOM TEMPERATURE

Sakinato Mazidda, Suyadi and Dyah Hikmawati

Abstract

This study aimed to determine the effect of antioxidants onion extract in Ringer’s Dextrose diluent on the quality of Gallus domesticus sperm during storage at room temperature. Onion extract was made using the maceration method with 50% ethanol as a solvent and evaporated with a vacuum rotary evaporator with a temperature of 50 ºC. Gallus domesticus sperm was collected using female teaser method. The collected semen was divided into 4 treatments, namely T0 (Ringer’s Dextrose without onion extract), T1 (Ringer’s Dextrose + 0.02 mL of onion extract), T2 (Ringer’s Dextrose + 0.04 mL of onion extract) and T3 (Ringer’s Dextrose + 0.06 mL of onion extract). Evaluation of semen quality during the storage at room temperature including individual motility, viability, and abnormality were observed within 1, 2, 3, and 4 hours. The experimental design used a Completely Randomized Design (CRD), the data analysis used Variance Analysis (ANOVA) and followed by Duncan’s Multiple Range Tests (DMRT). The analysis showed significant differences (P <0.01) for individual motility and (P <0.05) for sperm viability. The best results were shown in T2 (Ringer’s Dextrose + 0.04 mL of onion extract). The sperm quality resulted by T2 treatment could last up to 4 hours at room temperature, with an average of individual motility of 50.83 ± 3.76b, viability of 76.28 ± 2.66b and abnormality of 16.87 ± 1.24a. The addition of 0.04 mL onion extract in Ringer’s Dextrose diluent within 4 hours at room temperature met the quality requirements to be used in Artificial Insemination (AI).