PRELIMINARY STUDY OF DENGUE VIRUS SEROTYPE ON AEDES MOSQUITOES IN ENDEMIC AREA, SURABAYA, INDONESIA, JANUARY 2020Aulia Azzahra, Lucky Vera Oktavia, Muhammad Fariz Naviyanto, Shifa Fauziyah, Teguh Hari Sucipto, Dwi Winarni, Sri Puji Astutik Wahyuningsih, Siti Churrotin, Ilham Harlan Amarullah and Soegeng Soegijanto
Aedes sp. is a mosquito that can act as a vector of Dengue Haemorrhagic Fever (DHF). This mosquito can contain dengue virus (DENV) that comes from the blood of dengue sufferers. Dengue virus consists of 4 types of serotypes, namely DENV-1, DENV-2, DENV-3 and DENV-4. In this report, we observe the existence of Dengue virus in Aedes albopictus from Institute of Tropical Diseaseâs parking area. Our observation start from mosquito identification, preparation and RNA extraction, RNA purity test by Nanodrop Spectrophotometer, convert RNA become cDNA by Reverse TranscriptionâPolymerase Chain Reaction, DNA amplification using Polymerase Chain Reaction, electrophoresis, and visualize using UV transilluminator. The mosquitoes that had been collected identified as female Ae. albopictus based on their one thick white line on itâs mesonotum without two curved line and their thread-like antennae, with short and rare hair. Visualization by UV transilluminator present that Ae. albopictus contain DENV-2 dengue virus. In 2013, DENV-3 appeared when DENV-1 become predominant during 2008-2013. It was predicted that DENV-3 would be an outbreak in next year. In fact, it turned out that DENV-3 was really became an outbreak in 2013-2016. It indicated the possibility that DENV-2 we have just discovered could become an outbreak in the future.