Pollution Research Paper

Vol 39, Nov Suppl. Issue, 2020; Page No.(166-172)

MICROBIAL DEGRADATION OF NAPHTHALENE THROUGH BACILLUS CEREUS RD6 ISOLATED FROM REFINERY OIL SLUDGE

RUBY DOLEY AND MANOJ BARTHAKUR

Abstract

Oil contains a number of hydrocarbons. It is a complex mixture of linear, branched and cyclic alkanes, and mono- and polyaromatic compounds like benzene, toluene, xylene, ethylbenzene, naphthalene etc. These compounds are of utmost concern to human health because of their genotoxic, mutagenic and carcinogenic effects. Naphthalene is a common polycyclic aromatic hydrocarbon (PAH) and has been considered as a model for microbial degradation study. The aim of this study was to evaluate the naphthalene degradability by indigenous bacterial strain isolated from oil sludge. Twelve bacterial isolates were obtained from the oil sludge of Guwahati Refinery, Assam, India and after preliminary screening the isolate designated as RD6 was considered for degradation study of naphthalene. The isolate was further identified as Bacillus cereus through biochemical tests and molecular characterization by 16SrDNA gene sequencing. Phylogenetic tree was constructed with BLAST result of closely related sequences using MEGA 7 software. The sequence of Bacillus cereus RD6 (MH114968) was submitted to NCBI gene bank for global retrieval. Detection of eight metabolic biomarkers such as oxalic acid, dibutyl phthalate, 1,2- benzene dicarboxylic acid, fumaric acid, 2-propenoic acid, phthalic acid, Benzoic acid, and Eicosane in GC-MS analysis reveals the degradation of naphthalene by Bacillus cereus RD6.

Journal Issues
Vol 39, Nov Suppl. Issue, 2020
Vol 39, Issue 3, 2020
Vol 39, Issue 2, 2020
Vol 39, Issue 1, 2020
Vol 39, Feb Suppl. Issue, 2020
Vol 38, Issue 4, 2019
Vol 38, November Suppl Issue
Vol 38, Issue 3, 2019
Vol 38, August Suppl Issue
Vol 38, Issue 2, 2019
Vol 38, March Suppl Issue
Vol 38, Issue 1, 2019
Vol 37, Issue 4, 2018
Vol 37, Issue 3, 2018
Vol 37, Issue 2, 2018
Vol 37, May Suppl. Issue 2018
Vol 37, Issue 1, 2018
Vol. 36, Issue 4, 2017
Vol. 36, Issue 3, 2017
Vol. 36, Issue 2, 2017
Vol. 36, Issue 1, 2017
Vol. 35, Issue 4, 2016
Vol. 35, Issue 3, 2016
Vol. 35, Issue 2, 2016
Vol. 35, Issue 1, 2016
Vol 34, Issue 4, 2015
Vol 34, Issue 3, 2015
Vol 34, Issue 2, 2015
Vol 34, Issue 1, 2015
Vol 33, Issue 04, 2014
Vol 33, Issue 03, 2014
Vol 33, Issue 02, 2014
Vol.33, Issue 01, 2014
Vol.32, Issue 04, 2013
Vol.32, Issue 03, 2013
Vol.32, Issue 02, 2013
Vol.32, Issue 1, 2013
Vol.31, Issue 04, 2012
Vol.31, Issue 3, 2012
Vol.31, Issue 2, 2012
Vol.31, Issue 1, 2012
Vol.30, Issue 4, 2011
Vol.30, Issue 3, 2011
Vol.30, Issue 2, 2011
Vol.30, Issue 1, 2011
Vol.29, Issue 4, 2010
Vol.29, Issue 3, 2010
Vol.29, Issue 2, 2010
Vol.29, Issue 1, 2010
Vol.26, Issue 3, 2007
Vol.20, Issue 02, 2001
Vol.20, Issue 01, 2001
Vol.19, Issue 03, 2000
Vol.19, Issue 01, 2000
Vol.18, Issue 02, 1999
Vol.17, Issue 04, 1998
Vol.17, Issue 03, 1998
Vol.17, Issue 01, 1998
Looking for Past Issues? Click here to get them!!