Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 22, Issue 3, 2020; Page No.(486-490)

COST EFFECTIVE BIOMASS PRODUCTION OF CHLORELLA HOMOSPHAERA AND SCENEDESMUS OBLIQUUS-TWO BIOFUEL POTENT MICROALGAE FROM NORTHEAST INDIA

BANASREE SHARMA, PRIYANKA PAUL, ANUCHAYA DEVI, MOHAN CHANDRA KALITA, DHANAPATI DEKA AND VAIBHAB V. GOUD

Abstract

Microalgal strains are potent for algal lipid generation as well as biofuel production and on the other hand animal manure is a commonly found waste product in Assam which are rich in nitrogen and phosphorus inter alia and is vital for algal growth, therefore in the present study two trials of experiments were conducted to evaluate the biomass in terms of growth and biofuel potential of two indigenous freshwater microalgae Scenedesmus obliquus and Chlorella homosphaera in three different medium such as Cow dung extract, Goat droppings extract and BG-11 media under controlled laboratory growth conditions in different concentrations (2- 10%) and another set with Urea as an added nitrogen source. It was observed that among the three media at different concentrations Chlorella homosphaera grows best in 10% of goat dropping extract achieving a specific growth rate of 0.504(μ/d) and lipid content of 31.5%, similarly, on the other hand, Scenedesmus obliquus also exhibited highest growth in 8% of goat dropping extract with added urea achieving an specific growth rate of 0.279(μ/d) and lipid content of 22%. 1HNMR analysis also confirms the presence of diglyceride and monoglyceride along with the triglyceride. Based on our study it can be said that goat dropping extract as organic media can be a cheap, simple and inexpensive alternative method by which more biomass and biofuel can be generated.

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